Recently multiple groups have compared the performance of current next-gen sequencing technologies. Independent comparisons of different next-gen sequencing machines have given us a good view of what one can expect from these constantly changing technologies.
Apart from performance, it is also good to know about price and tech. spec. differences – a much easier aspects of next-gen sequencing technology comparisons. As smaller labs have started thinking about using next-gen sequencing , learning about the costs and tech specs of different sequencing technologies is often the first step. Here is such comparison from two recent publications.
Loman et. al and Quail et. al. have presented a nice comparison of price and technical specifications for the current next-gen sequencing machines by sequencing bacterial genomes. Both Loman et. al and Quail et. al compared the next-gen sequencing technologies available as of Dec. 2011. For sure, some aspects of the price/tech. spec comparison will be slightly different. Here is a brief summary of the pricing and sequencing capability comparisons done by Loman et. al. and Quail. et. al.
Price and Sequencing Capability Comparison of 454 GS Junior, Ion Torrent PGM, and Illumina MiSeq
Loman et. al. performed a detailed comparison of 454 GS Junior, Ion Torrent PGM, and Illumina MiSeq- the current benchtop next-gen sequencers by sequencing E.Coli. If you are interested in the summary of their findings, visit
|Next-Gen Sequencer||Machine Cost||Cost per run||Minimum Throughput||Sequencing Run Time||Cost Per Mb|
|Illumina MiSeq||$125,000||$750||1500 Mb (2 x 150 Bases)||27 Hours||$0.5|
|454 GS Junior||$108,000||$1,100||35 Mb (400 Bases)||8 Hours||$31|
|Ion Torrent PGM - 314 Chip||$80,490||$225||10Mb (100 Bases)||3 Hours||$22.5|
|Ion Torrent PGM - 316 Chip||$80,490||$425||100Mb||3 Hours||$4.25|
|Ion Torrent PGM - 318 Chip||$80,490||$625||1000Mb||3 Hours||$0.63|
Price and Sequencing Capability Comparison of PacBio RS, Ion Torrent PGM, and Illumina MiSeq
|Machine Cost||Throughput Per Run||Sequencing Cost per Gb||Sequencing Run Time||Observed Error rate|
|Illumina MiSeq||$128,000||1.5-2 Gb||$502||27 Hours||0.8%|
|PacBio RS||$695,000||100 Mb||$2000||2 Hours||12.86%|
|Ion Torrent -318 Chip||$80,000||1Gb||$1000||2 Hours||1.71%|
|Illumina GAIIx||$256,000||30 Gb||$148||10 Days||0.76%|
|Illumina HiSeq 2000||$654,000||600 Gb||$41||11 Days||0.26%|