Illumina added Moleculo Technology to its technology page on its website. Illumina acquired the San Francisco based startup company founded by Stephen quake’s members Michael Kertesz and Dmitry Pushkarev in late 2012. All one initially knew was Moleculo Technology offers long reads using existing Illumina sequencers like HiSeq. After Plant and Animal Genomes conference, a bit more details emerged on what Moleculo Technology could do. Now, Illumina gives some details on Moleculo technology for the first time.
Moleculo Technology combines a new sample preparation method with informatics to generate long synthetic reads using Illumina HiSeq machines. Illumina’ technology page says that the first step of the Moleculo technology is to break genomic DNA into large fragments of sizes 10 kb. Then these 10 kb fragments are then amplified, sheared, and barcode attached. These fragments were then sequenced on Illumina technology as short sequence reads. The short sequence reads were then assembled into genomic fragments computationally. At the end of it one gets a full sequence of all the fragments reconstructed from short?read data.
Illumina says that, the Sample prep for the Moleculo technology takes about 2 days without the sequencing time. Using Moleculo Technology, Illumina could to phase a human genome sequenced on the HiSeq 2500 in less than 4 days using Moleculo’s phasing algorithm.
Illumina hopes that the Moleculo Technology has the potential to improve
the efficiency and accuracy of many existing DNA sequencing applications while increasing the coverage of clinically important genes. These advantages allow for the phased resequencing of human genomes and rapid de novosequencing of plant and animal genomes.
Curious to learn more about Moleculo? Read the Bio IT World’s interview “Moleculo Man: Mickey Kertesz on Illumina’s Sub-Assembly Acquisition“