Illumina Announces Moleculo Long Read Technology and Phasing As Service

Illumina kept its promise of making Moleculo’s Long Read Technoogy as service in 2013.  Illumina announced today that Illumina’s FastTrack Services will be offering Long-Read Sequencing Services and new Phasing Analysis. With the two new additional services, Illumina can provide whole-genome results within twelve weeks. Illumina’s Long Read sequencing service is not only available for human, but for any organism with genome size bigger than 100Mb.

Illumina’s Long Read Sequencing technology needs 1 µg of genomic DNA and can produce fragments of size up to 10 kilobases. A single library produces about 600Mb of long read sequence data. Announcing the long read sequencing as service, Illumina also said that the Long Read sequencing technology will be soon available as a kit.

If you are interested in learning more about the Long Read sequencing technology, check out the first publication from Stephen Quake’s group describing Moleculo Long Read Sequencing technology as LRSeq and applying to sequence the genome of a chordate, The genome sequence of the colonial chordate, Botryllus schlosseri.  llumina has also made available some long read sequence data from Fly genome in its Basespace.

The paper also revealed the patents, US and International patent application numbers 61/532,882 and 13/608,778 entitled “Methods for obtaining a sequence”, behind the LRSeq technology.

LRSeq-Moleculo-eLife

LRSeq-Moleculo-eLife

The paper published in early July gives more detail on the Moleculo/LRSeq technology

Our approach began with genomic DNA sheared to 6–8 kb fragments. Limiting dilution was used to create aliquots of a few hundred to a few thousand DNA molecules. Each aliquot was amplified with PCR, fragmented (600–800 bp), barcoded, and sequenced by Illumina HiSeq 2000 (Figure 2). The Velvet assembler (Zerbino, 2010) was used to assemble short paired-end reads from each barcode (i.e., well) separately, thus simplifying the assembly problem and creating effective read lengths corresponding to the original large fragment sizes (Figure 2B; Supplementary file 2A, Supplementary file 2B). Limiting the number of DNA molecules per well greatly reduces or eliminates chances of having a repeated or duplicate sequence within a defined partition. Furthermore, since each well was over-sequenced, the error rate is reduced by the coverage and is substantially improved from the intrinsic error rate of the sequencer (Supplementary file 2C). This procedure is amenable to automation in multiwell plates, and we obtained data from twelve 96-well plates (Supplementary file 2A, Supplementary file 2B).

 

Trackbacks

  1. […] possible Moleculo data is from the publication first publication using Moleculo technology.  The Moleculo team worked on the project before naming the technology […]

  2. […] but much improved, to LR-Seq, an earlier version used to sequence B. schlosseri by Moleculo (published in eLife last year). One of the biggest concern with Moleculo long read technology is the bias from PCR amplification. […]

Speak Your Mind

*